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    The International Society for Aerosols in Medicine

    Arno Gutleb

    Arno Gutleb

    Prof. Arno Gutlab

    Luxembourg Institute of Science and Technology (LIST)
    Luxembourg

    Prof. Arno Gutleb is a European registered toxicologist (ERT). He is Group Leader Environmental Health at the Luxembourg Institute of Science and Technology and method development for the toxicity testing of the future especially for the alveolar barrier is the core activity of his group.


    3D-in vitro alveolar models – State-of-the-art!

    Abstract
    Air-liquid interface in vitro lung cell models can be used to investigate physiological and pathophysiological responses, molecular events and modes of action and interaction of different cell types of the respiratory tract. Potentially, they mimic more closely the in vivo situation of cells in the respiratory tract, namely being apically exposed to air. Such air-liquid interface models focus on particular anatomical regions of the lung or particular molecular pathways and aim to model processes that are relevant in vivo. Interest in such models has grown since they can provide information on the effects of therapeutics, xenobiotics, particulate matter etc. in lungs and potentially downstream systemic consequences that may relate to health and disease specific end-points. Animals have routinely been used for such assessments simply because no in vitro methods have ever been validated to sufficient levels. In that respect, animal usage in this domain is still widely seen as the only option available. However, anatomical differences between rodent and human lungs are significant, which has strong implications for the deposition rates of particles and chemicals making extrapolation from animals to humans difficult. There is a strong societal and legal support to reduce, refine and replace the use of animals and this in turn is driving the development of so-called alternative methods – the most promising being air-liquid interface approaches in the domain of inhalation toxicology.